Environmental Monitoring, Analytical Testing Laboratory at the Golden LEAF Biomanufacturing Training and Education Center (BTEC), North Carolina State University
Area of research and sub-discipline:
Microbial and Endotoxin testing of the High Purity Water system at the BTEC, North Carolina State University
About the lab:
NC State University’s Golden LEAF BTEC is helping to solve the biomanufacturing industry’s toughest scientific problems. In addition to providing undergraduate, graduate and professional education and training, BTEC offers scientists from industry, government and academia access to its facilities, equipment and the expertise of its faculty and staff. In BTEC’s analytical lab, technologies include HPLC, UPLC, ELISA, gel electrophoresis, capillary electrophoresis, MALDI-TOF, LC-MS, GC-MS and quantitative PCR. The lab offers routine analytical testing services, assay development and validation services. Two typical tests performed by the lab include the test for the presence of Endotoxin and Bioburden.
An introductory biology (or microbiology course) and basic laboratory skills would be useful, but not required.
Specific project(s) available:
The BTEC is equipped with a host of support systems required for biopharmaceutical production, including clean steam and high purity water (HPW). The analytical laboratory has the capabilities of testing the HPW for the presence of endotoxin and bioburden, two prevalent viable environmental contaminants through the LAL gel clot method limit test (USP 34 Chapter 85) and the Milliflex membrane filtration system, respectively. This project involves monitoring the BTEC HPW systems. The student would train in the above mentioned endotoxin and bioburden assays to become proficient, sample the high purity water supply at key facility locations, analyze and report the results, and author a standard operating procedure for monitoring the HPW on a routine basis. As time allows, the student can also be trained in microbial ID assays to identify the microbes found in the water supply. This opportunity is available for students attending Saint Augustine’s University.